The 2001 Tuskegee Golden Tigers football team represented Tuskegee University as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 2001 NCAA Division II football season. Led by sixth-year head coach Rick Comegy, the Golden Tigers compiled an overall record of 11–1, with a conference record of 6–1, and finished as SIAC co-champion. At the conclusion of the season, the Golden Tigers were also recognized as black college national champion.
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